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The Purity Test of the Major Compound Isolated from the Green and Yellow Kayu Angin (Usnea sp) (Uji Kemurnian Senyawa Utama yang Diisolasi dari Kayu Angin (Usnea sp.) Hijau dan Kuning)

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Kayu angin is a liken that grows in Indonesia forests and commonly used to cure diarhea, stomachache, and cough. Based on the densitometric method, yellow kayu angin (KAK) contained 4.09 percent (from the dry weight) of major substance (substance A), and 2.99 percent of major substance (substance B) from green kayu angin (KAH). Previously, these substances were identified as uric acid which inhibits the growth os Staphylococcus aureus B (5 ug/ml). Therefore it is important to isolate A and B in a large scale, analyze the impurities and identify these substances. Isolation of substances A and B was performed with aseton in soxhlet. The resulting aseton extract was evaporated to dryness and the residu was re-extracted with chloroform to give precipitate and a soluble fraction which contained the major substance. Upon evaporating the chloroform soluble fraction followed by crystalization and recrystalization of the residu with aseton, a yellow crystalline having melting point of 206 degree-207 degree C was obtained. The impurity of subtances A and B was checked by a hplc method. The hplc chromatogram indicated that A consisted of 6 peaks, 3 of them are major components; while the B consists of 13 components and similary B has 3 major components. One of the three major components of A showed an identical retention time (28.667 minutes) with that of B, which indicated that A and B probably shared one similar compound. The three major components present in A were identified as (+) uric acid (42.95 percent), stictine acid and salazin acid; while those three components present in B were identified as (+) uric acid (36.99 percent), norstictine acid and unidentified stictine acid derivative.

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Majalah Farmasi Indonesia,6(1)1995:1-7

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: ., 1995

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plant extracts

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