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Sensitivity of Rapid Test for Detection of Hepatitis Surface Antigen (HBsAg) in Patients with Hepatitis B Virus (Sensitivitas Rapid Test untuk Mendeteksi Hepatitis Surface Antigen (HBsAg) pada Penderita Hepatitis B.)

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A new rapid-test, sandwich immunochromatography assay technique has been recently introduced for the routine, rapid, qualitative detection of the resence hepatitis-B surface antigen (HBsAG). To evaluate the sensitivity of rapid test, we studied 39 sera positive and one sera negative for HBsAg with conventional ELISA method. The study found that the minimal detectable level of HBsAg with rapid test was 4.03 mg/ml (range 3.25-4.03 ng/ml). It was 8 times less sensitive than conventional ELISA method (4.03 vs 0.47 ng/ml). Many factors such as 'eddy diffusion' and structure of HBsAg, humidity, direct contact with sunlight and sera of borderline-low level of HBaAg with conventional ELISA had a potency for lower sensitivity of rapid test; conversely, technical factors such as adhesion, migration and viscosity of the samples do not.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Berkala Ilmu Kedokteran,28(3)1996:109-114

Penerbit

: ., 1996

Deskripsi Fisik

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Bahasa

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

hepatitis b virus

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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