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The Effect of Vitamin C on Fibroblast Proliferation and VEGF Expression in Fibroblast Culture

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One of the factors that determine the success effficien wound healing is wound healing is wound healing rate, that can be achieved by increasing cell proliferation, angiogenesis or neovascularisation, and extracellular production . Dermal fibroblast is cell that plays an important role in wound healing. Fibroblast proliferation and neovascularisation are critical elements in granular tissue formation. Local hypoxia causes fibroblast to express HIF-1 a that will induce fibroblast VEGF expression. The nature of vitamin C makes it easily oxidized. The addition of vitamin C on fibroblast culture medium is expected to produce local hypoxia condition that will induce fibroblast expression of HIF-1a, so that the expression of fibroblast VEGF will be increased. Vitamin C may modulate the growth of various types of cells. The effect of vitamin C on normal fibroblast proliferation and fibroblast VEGF expression is still unknown. This study was aimed to know whether vitamin C can increase normal human fibroblast proliferation and expression of VEGF.A simple experimental study was conducted by using preputial skin fibroblast culture from 10-year-old donor, subculture passage 3. Fibroblast culture was divided into 6 groups, each group received vitamin C treatment with the dose of 500ug/mL, 100ug/mL, 150 ug/mL, 200 ug/mL, and 300 ug/mL, and one group without treatment acting as control. Measurement of fibroblast proliferation wa conducted by spectrophotometer using MTT, and fibroblast expression of VEGF was measured using ELISA. The average of difference in fibroblast proliferation and VEGF expression was analyzed with one-way analysis of variance. There was a significant increase in fibroblast proliferation rate in the groups receiving vitamin C with the dose of 200 mg/mL (p=0.016) and 300 mg/mL (p=0.005), whereas in the group with the dose of 50 mg/mL, 100 mg/Ml and 150 mg/mL there was no significant difference compared to the control (p=0.933, p=0.961, p=0.301, respectively).Average fibroblast VEGF expression between various concentrations of vitamin C compared to the control showed no significant difference (p>0.05) Vitamin C could beconsidered to be an agent to accelerate wounds healing.

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Series Title

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Call Number

Berkala Ilmu Kedokteran, 41(3): 152-156

Publisher

Yogyakarta : FK. Universitas Gadjah Mada., 2009

Collation

4p.

Language

English

ISBN/ISSN

0125-9326

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Edition

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Subject(s)

ASCOBIC ACID
VASCULAR ENDOTHELIAL GRWTH FACTORS

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