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The Effect of Pandanus Conoidue Lam. Oil on Pancreatic beta-cells and Glibenclaide Hypoglycemic Effect of Diabetic Wistar Rats.

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Oxidative stress has been implicated in pathogenesis and progression of many degenerative disorders including naturally occurring and chemically-induced diabetes mellitus. In addition to the increased production of free radicals, antioxidant defense systems are disturbed in diabetes mellitus. As a result, oxidative stress may cause oxidative damage of cellular, including ß-cells of pancreas. This study was aimed at investigating the effect of P. condeus Lam, on hypoglycemic activity of glibenclamie and histochemical changes in pancreatic islets. Thirty male Rattus norvegicus rats were used in the study, and randomly selected. The animals were divided into two groups, each group consisted of 15 rats. Each group was devided into 3 subgroups, the first subgroups were subgroup 1,2,3 and the the second was subgroup 2,5,6> Streptozotocin was injected intraperitoneally at single dose of 60 mg/kg for induction of duiabetes in the second group. Subdroup 1 and 4 did not receive anything, subgroup 2 and 5 received glibenclamide 0.09g/kgBW/day and subgroup 3 and 6 received P. conideus Lam. O.3 mL/kgBW/day and glibenclamide 0.09 kgBW/day. Rats were given P. conoideus lam, and glibenclamide everyday for 14 days. Hypoglycemic activity was evaluated in overnight fasted at day 1, 7 and 14, and the acute hypoglycemic activity was evaluated at time 0, 1, 2, 4, and 6 hour after treatment at day 14. Blood was collected from orbital sinuses. Blood glucose level was determined using GOD-PAP method. At 15 day rat were killed, the pancreas was removed. The data was compared statistically using one-way analysis of variance (ANOVA) followed by post-hoc test and student unpaired t-test. For the analysis of immunohistochemical data, a non parametric test (Kruskal-Wallis) was used. The combination of P. conoideus Lam, and glibenclamide produced significant reduction in blood glucose in the serial study at day 1,7and 14 compareble with that of standard drug, glibenclamide also control groups in normal and diabetic rats. It also produced significant of blood glucoce in the serial study at time 0,1,2,4, and 6 hour after treatment at day 14, significant increasing histoscore, number and diameter of Langerhans islets were observed in P. conoides lam, treated rats .

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Detail Information

Series Title

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Call Number

Berkala Ilmu Kedokteran, 41(1):11-19

Publisher

Yogyakarta : FKUniversitasGadjah mada., 2009

Collation

9p.

Language

English

ISBN/ISSN

0126-1312

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

plants, medicinal
PANCREAS
GLYBUOIDE
HYPOGLYCEMIA

Specific Detail Info

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Statement of Responsibility

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