Nilai Diagnostik Pemeriksaan Reaksi Rantai Polimerase pada Tuberkulosis Paru Sputum Basil Tahan Asam Negatf | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Nilai Diagnostik Pemeriksaan Reaksi Rantai Polimerase pada Tuberkulosis Paru Sputum Basil Tahan Asam Negatf

I Wayan Agus Putra - Personal Name; Edy Surjanto - Personal Name; Suradi - Personal Name; Tjandra Yoga Aditama - Personal Name;

Tuberculosis (TB) remans an important health problem in Indonesia. Diagnosis of pulmonary tuberculosis reqires sputum examination and isolation of M. tuberculosis from a culture specimen. The limitation of acid-fast bacilli (AFB) micropic has relatively low sensitivity and solid media culture of M. tuberculosis required for long time (4-6 weeks) indentifying M. tuberculosis Approximately 30-60% of pulmonary tuberculosis recorded negative AFB. Thus patients with negative AFB were usually clinical and unsepcified radiological. Pulmomerase Chain Reaction (PCR) method amplifying DNA or RNA in vitro were the method uses as detection, identification and snsitivity test Mycabacterium tuberculosis. The aim of this study was to determine the valdity of PR for the diagnosis of pulmonary tuberculosis negative AFB and to obtain the method more rapid for detection of M. tuberculosis that than solid media Kudoh. A cross sectional study was conducte in Moewardi General Hospital Surakarta. Sevent four patients of pulmonary TB with negative AFB were requited in this study. All sputum speciamens were examined on the solid media (Kudon) and PCR method. All patients were given antituberculosis drug. Respond of the therapy would be analysed in the folowing 2 months. We got 26 patients positive culture media solid Kudoh and 61 patients positive PCR from the sample of 74 speciments with pulmonary tuberculosis negative AFB. The sensitivity and specificity of PCR on the solid media Kudoh were 96% and 25%. PCR o clinical responds were 84.7% and 10%. The conclusion of this study were the sensitivity of PCR method for the diagnosis of pulmonary tubercuosis negative AFB relatively high (96%) bt not specificity (25%).

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Location name is not set Jurnal Respirologi Indonesia, 28(3):138-144

A00001884

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Series Title: -
Call Number: Jurnal Respirologi Indonesia, 28(3):138-144
Publisher: Jakarta : Perhimpunan Dokter Paru Indonesia., 2008
Collation: 7P.
Language
ISBN/ISSN: 0853-7704
Classification: -
Content Type: -
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Edition: -
Subject(s): tuberculosis, pulmonary
polymerase chain reaction
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