Text

Perbedaan Jumlah Sel Darah pada Urine Segar dan Preservasi

---

To prevent lyses of the blood cells that consist of erythrocyte and leukocyte in urine. It is necessary to preserve it by storing it at 4ºC or giving 37% formalin. Therefore, one group post design experimental study was conducted to confirm the influence of preservation toward the number of blood cells in urine. The objective of this study was to verify the influence of 3-hour storage at room temperature, at room temperature +37% formalin, and at 4ºC to the number of blood cells in urine, and to prove that 3 ways of preservation give a different influence to the number of blood cells. Samples were taken from 10 different patients, every sample underwent 4 treatments. Urine 1 was kept fresh, urine 2 was kept for 3 hours at room temperature, urine 3 was kept for 3 hours at room temperature + 37% formalin, urine 4 was kept at 40ºC. After being centrifuged, the blood cells were examined microscopically using counting chamber, where the data obtained were analyzed using Anova Repeat Measure statistical test. Results of the study showed that there was a significant difference of the number of erythrocytes at room temperature (p=0,017), at 4ºC (p=0,017) and at room temperature +37% formalin (p=0,029), but from the three different ways of preservation, the study showed that samples kept at room temperature +37% formalin gave the smallest difference (d=166,700). It was also shown, there was a significant difference of the number of leukocytes at room temperature and at 40º C (p=0,004), and there was no significant difference of the number of leukocytes at room temperature +37% formalin (p=0,065). Based on the data, the study conclude that preservation at room temperature, room temperature +37% formalin, and 40ºC caused significant decrease of the number of erythrocytes, but preservation at room temperature + 37% formalin gave the smallest decrease. Whereas preservation at room temperature and 4ºC caused significant decrease of the number of leukocytes, but preservation at room temperature + 37% formalin caused no significant decrease. So, the use of 37% formalin is the best preservation method.

---

Availability

Detail Information

Series Title

-

Call Number

Jurnal Politeknik Penelitian Kesehatan: 6(1):32-40

Publisher

Surabaya : Politeknik Kesehatan Surabaya., 2008

Collation

10p.

Language

ISBN/ISSN

1693-3753

Classification

-

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

URINE
BLOOD CELLS

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment