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Efek Imunomodulator Propolis pada Fagositosis Intraselluler

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The study investigated immmunomodulator effects of propolis on mice peritoneal macrophage phagocytosis against Salmonella typhi. Peritoneal macrophage cells were obtained from male BALB/c weighing approximately 30 grams, between 6 and 8 weeks old, which were previously injected with 10 ml of PBS into their abdominal cavities. After a soft abdominal massage for 1 minute, the peritoneal liquid was collected with intravenous cathether and was put in a plastic tube. The liquid was then devided into non-propolis and propolis group. The non-propolis group was not given propolis and the group acts as control group, while the propolis group was given 0,7 µl of propolis., each group was given Salmonella typhi suspension equivalent to 3 x 108 CFU/ml Mc Farland. The number of bacteria per macrophage and the number of macrophage that contains bacteria in the time scale of 30, 60, 90, 120 seconds were counted. For in vitro phagocytosis tests the ratio of macrophage to bacteria is 106 : 107. The activity of peritoneal macrophage intracellular phagocytosis was visualized using double staining microscopy technique, which exhibited a clear distinction between extracellular and intracellular bacteria. The study was structured using factorial design (2x4). The factors consisted of propolis [dose] adjustment and exposure time. The formulated hypothesisnwas examined nusing Multivariate Test and Univariate Analysis of Variance, followed by F test, at the significance level of 0.05. The result showed that the propolis have increased the macrophage phagocytosis capacity significantly, by 2.65 fold, against intracellular bacteria such as Salmonella typhi.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Jurnal Politeknik Penelitian Kesehatan: 6(1):6-10

Penerbit

Surabaya : Politeknik Kesehatan Surabaya., 2008

Deskripsi Fisik

5p.

Bahasa

ISBN/ISSN

1693-3753

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

salmonella typhi
IMMUNOLOGIC FACTORS
propolis

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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