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Adiponectin Down Regulated Inducible Nitrit Oxide (iNOS) Expression on Huvec's Culture Treated with Lipolysaccharide (LPS)

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Adiponectin is a adiocyte derivate protein involves in sensitivity of insulin, anti-inflamation, and antiatherogenic found largely in plasma. The decrease of adiponectin in the plasma is related to obesity, cardiovascular diseases, type II diabetes, hypertension and vascular injuries in nice. In vivo research on strain balb/c mice showed that excessive Adiponectin expression inhibits atherosclerosis with Apo E deficiency. The aim of this research is to find protective mechanisms of adiponectin on vascular utilizing invitro Infected HUVEC’s culture. There were 5 groups: (A) control negative group of normal HUVEC’s , (B) control positive group or HUVEC’s treated 10 ug/ml LPS, (C) HUVEC’s treated with 10 ug/ml LPS and adiponectin 0,001 ng/ml, (D) HUVEC’s LPS and adiponectin 0,1 ng/ml, (E) HUVEC’s treated with 10 ug/ml LPS and adiponectin 1 ng/ml. This study used CISH (chromogenic in Situ Hybrization) technique to observe mRNA iNOS expression with 27 bases sequences (5”-accttaaggcagcct-gtgagcgtt-3”). The control group did not show mRNA iNOS expression. The iNOS expression increased with LPS Induction, however adiponectin treatment on HUVEC’s culture cells decreased of mRA iNOS Expression the regulation can be proof by adding antibody to iNOS protein of 24 hours adeponectin treated culture. This study describes that the injection of adiponectin down regulates the expression of iNOS endothelial that treated with LPS. This means that adiponectin may have role in regulates the expression of iNOS endothelial that treated with LPS. This means that adiponectin may role in regulating the decrease on iNOS expression.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Jurnal Kedokteran Brawijaya,24(3):106-110

Penerbit

Malang : Fk. Universitas Brawijaya., 2008

Deskripsi Fisik

3p.

Bahasa

ISBN/ISSN

0216-9347

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOCHEMISTRY
ADIPONECTIN
LIPOPOLYSACCHARIDES

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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