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Apllication of Non-Specific Esterase Enzyme Microassays to Detect Potential Insecticide Resistance of Aedes aegypti Adults in Yogyakarta, Indonesia

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By bioassay Aedes aegypty larvae collected from Yogyakarta were found to be susceptible to temphos and malathion and by using microplate nzymatic assay Ae. aegypti larvae collected from the same sites were potentially resistant to the organoposphate insecticides due to elevated esterase activity in hydrolyzing alpha-naphthyl acetate substrate used in the enzymatic reaction from the same sites might be potentially resistant to OP insecticides due to the same mechanism. The homoenates of the mosquito adult stages from the fields were microassayed using alpha-naphtyl acetate substrate compared to that colonized in the laboratory. From a series of studies the results were concluded that Ae. aegypti adults in Yogyakarta were susceptible and potentially resistant to insecticides due to elevated alpha-napthyl acetate esterase activity.

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Berkala Ilmu Kedokteran,28(4)1996:167-171

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: ., 1996

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aedes
insecticides

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