Loss of Hemterozygosity Analysis of RAR Beta 2 Gene in the Pathogenesis of Breast Cancer | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Loss of Hemterozygosity Analysis of RAR Beta 2 Gene in the Pathogenesis of Breast Cancer

I Ketut Gede muliartha - Personal Name; Wibi Riawan - Personal Name; Asnah Hidayati - Personal Name; Fitri Armania - Personal Name;

The aim of this research was to investigate the event of loss of heterozygosity of retinoid acid receptor (PAR ß2) gene on the chromosom 3024 in the pathogenesis of breast cancer. The breast cancer is the most malignant tumor that leads the second cause of death death among the female. The recent concept of the theory of pathogenesis breast cancer is molecular genetic approaches such as apoptosis gene, oncogen, suppressor gene, and growth promoting fator gene. However, the diagnostic and therapy of breast cancer are still not satisfied. The lost or decreased in the expression of PAR ß2 gene on 3p24 chromosome via loss of heterozygosity (LOH) mechanism are the role of pathogenesis of breast cancer. Design of the research is by experimental and observational. Human female breast cancer and blood samples were gained from public hospital RSUD dr. Saiful Anwar, Malang. DNA was extracted from lymphocyte and tissue using Macherey-Nagel system kit. Loss of heterozygosity (LOH) incident I 3p24 chromosome was catected by PCR and chromogenic in situ hybridization (CISH) method. Result showed that LOH event has detected in chromosome 3p24 with the absence of several band of DNA by PCR. The LOH event also expressed in breast cancer tissues through the reduction of RAR ß2 expression as the grade of breast cancer increase. RAR ß2 gene is located at chromosome 3p24, and LOH event in chromosome 3p24 is common in breast cancer.

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Series Title: -
Call Number: Jurnal Kedokteran Brawijaya, 24(2): 86-89
Publisher: Mataram : Fk. Universitas Brawijaya., 2008
Collation: 3p.
Language
ISBN/ISSN: 0216-9347
Classification: -
Content Type: -
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Edition: -
Subject(s): breast neoplasms
LOSS OF HEFEROZYGOSITY
VIROLENCE
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