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Diagnosis of Malayan filariasis Using Sandwich ELISA and Monoclonal Antibody (Diagnosis Filariasis Malayi dengan ELISA Sandwich Menggunakan Antibodi Monoklonal )

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Malayan filariasis is still the major public health problem in Indonesia. In endemic area, when microfilaremia and clinical manifestations are absent, the diagnosis of malayan filariasis is extremely important, particurlarly for filariasis control. With the development of hybridoma technology, it is possible to produce monoclonal antibody against any stage of brugia malayi, which is capable of detecting the circulating parasite antigen in malayan filarisasis sera. Sandwich ELISA assay using Feb 4 monoclonal antibody could identify circulating parasite antigen in human sera. With this assay it was found that: 100 percent of sera from symptomatic-microfilaremic, 50 percent of sera from asymptomatic-amicrofilaremic residents of filariasis-endemic area positively contained circulating antigen. In comparison with the conventional method, the sensitivity and specificity of this new technique antigen. In comparison with the conventional method, the sensitivity of this new technique are 85.2 percent and 75.4 percent respectively. From this study it is concluded that Sandwich ELISA is proved to be very sensitive as an immunnological test to detect filarial infection without any clinical manifestation or microfilaria in the blood.

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Berkala Ilmu Kedokteran,28(2)1996:61-65

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: ., 1996

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filariasis
enzyme-linked immunosorbent assay

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