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Effect of Nitrogen Sources in Medium on the Growth and Sapogenins Production in Callus Cultures of Agave Amaniensis Trel Nowell
The effect of the nutrient nitrogen sources ammonium nitrate, ammonium chloride and potassium nitrate in the media, on the biomass and sapogenius production in the callus cultures of Agave amaniensis were studied. Combining the nutrate and ammonium ions in the media, produced relatively higher biomass, whilst using ammonium or nitrate ions only seemed to inhibit the growth of these callus cultures. The highest sapogenins production was achieved by using 4950 mg/l amonium ions as the nitrogen sources, produced relatively lower sapogenins.
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