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Aim This study analyze the MnSOD gene expression as endogenous antioxidant in human glioma cells compared with leucocyte cells as control. Methods MnSOD gene expression of20 glioma patients was analyzed by measuring the relative expression ofmRNA and enzyme activity of MnSOD in brain and leucocyte cells. The relative expression of mRNA MnSOD was deter¬mined by using quantitative Real Time RT-…
Aim To construct tissue microarrays (TMAs) that consisted of prostate tumours from the transgenic adenocarcinoma of mouse prostate (TRAMP) mice and non-transgenic murine prostates and to assess androgen receptor (AR) levels during progression of prostate cancer in TRAMP mice by immunohistochemistry. Methods Haematoxylin and eosin (H&E) sections from the ventral and dorso-lateral prostate lobes…
Captopril Is one of the most frequently used medicine In the treatment of hypertension with repeatedly used frequency in a day. Therefore captopril should be formulated in the form of sustained release and find the optimum formula. The purpose of this study was to determine the Influence of both factors and their interactions, which are the ratio of polymer HPMC K4M - xanthan gum factor at the …
Previous reasearch reported that chloroform extract that was partitioned from etanol exctracts of R. mucronata stem bark had sitotoxic against Myeloma cells (Diastuti, 2008). This research was aimed to fractinate the chloroform extract of R. mucronata stem bark and cytotoxicity test againts Myeloma cells, then identification the cytotoxic compounds. The ethanol extracts of R. mucronata steam ba…
Andrographolide, a bioactive component of Andrographis panicuiata (Burm.F) Nees, is the major lactone diterpenoidal bicyclic constituent in this plant which has proven to exert anti-inflammatory activity in vitro which was occurred via several mechanism, e.g inhibition of inducible nitric oxide synthase (iNOS), inhibition of radical oxygen species, and inhibition of NF-kappaB activation, The la…
Controlled delivery systems of a predetermined dose over a sustained period have been used to overcome the shortcomings of conventio.nal dosage forms. This is because the controlled drug delivery system can provide sustained therapeutic level of drug concentration without toxicity and convenience for patients, It would be more beneficial and ideal if the drug could be delivered by a device that…
Tetrahydropentagamavunon-O (THPGV-O) is assumed to be main metabolite product of biotransformation process of PGV-O. THPGV-O was synthesized by converting PGV-O to the compound by hydrogenation with PdjC as a catalyst. PGV-O potently inhibited the histamine release from ~at mast cells in vitro and in vivo, however, ironically only traces amount of compound was found in the blood. THPGV-O is ass…
The antibacterial activity of ethanolic extract of leaves and bulbs of Crinum asiaticum L. was tested against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis, pathogenic bacteria that cause acne. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by dilution methods. MIC and MBC of ethanol leaves extract were found f…
Malaria is a life-threatening disease caused by Plasmodium parasites. The rapid spread of malaria-quinoline resistance enforce to find of new antlmalaria drug. Artemisia annua L having artemlslnln as secondary metabolltes, has been used as antlmalarla agent for long time. One of source of bioactive compound Is endophytic fungus. This fungus can produce similar bioactive compound to host plant. …