Detenmination of total tannin of Sambang darah (Exoecaria bicolor Hasak) leaf, and jati belanda (Guazuma Ulmifolia Lamk) leaf colorimetric method using prusian blue reagent have been carried out. Total tannin of jati belanda leaf and sambaing darah leaf were 0.0138±0.0002% and 0.0173±0.0002%. The validation parameter using tannic acid as refence material gave correlation coefficient ®=0,9982…