Hybridization temperature of PCR technique plays an important role in obtaining the successful amplification of templates DNA. The hybridization temperature depends on the composition and the number of oligonucleotides existing in the primers. In this work we have optimized the hybridization temperature of seven primers used to amplify the templete DNA. The following methods were applied in thi…
Hybridization temperature of PCR technique plays an important role in obtaining the successful amplification of templates DNA. The hybridization temperature depends on the composition and the number of oligonucleotides existing in the primers. In this work we have optimized the hybridization temperature of seven primers used to amplify the templete DNA. The following methods were applied in thi…