Finding new substances from natural resources is quite important, especially those possessing medical usage. This experiment was perfomed in order to isolate and elucidate the stucture of pentacyclic triterpene and sterol substances from Eupatorium in lifolium H.B.K. leaves. The structural elucidation of those substances was performed using IR, UV, mass spectrometer 1H and 13C NMR. The result s…